Amplification of GC-rich genes by following a combination strategy of primer design, enhancers and modified PCR cycle conditions. Sahdev, S., Saini, S., Tiwari, P., Saxena, S. Betaine, dimethyl sulfoxide, and 7-deaza-dGTP, a powerful mixture for amplification of GC-rich DNA sequences. Musso, M., Bocciardi, R., Parodi, S., Ravazzolo, R. Improved PCR method for amplification of GC-rich DNA sequences. Optimization of the annealing temperature for DNA amplification in vitro. The highest gene concentrations in the human genome are in telomeric bands of metaphase chromosomes.
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Comparison of manual and automated nucleic acid extraction from whole-blood samples. Association of a novel regulatory polymorphism (-938C>A) in the BCL2 gene promoter with disease progression and survival in chronic lymphocytic leukemia. The AA genotype of the regulatory BCL2 promoter polymorphism (938C>A) is associated with a favorable outcome in lymph node negative invasive breast cancer patients. Characterization of the GNAQ promoter and association of increased Gq expression with cardiac hypertrophy in humans. A novel promoter polymorphism in the human gene GNAS affects binding of transcription factor upstream stimulatory factor 1, Galphas protein expression and body weight regulation. Successful amplification of extremely GC-rich promoter regions using a novel 'slowdown PCR' technique. Regulation of gene expression by GC-rich DNA cis-elements. Denaturants or cosolvents improve the specificity of PCR amplification of a G + C-rich DNA using genetically engineered DNA polymerases. Localised sequence regions possessing high melting temperatures prevent the amplification of a DNA mimic in competitive PCR. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
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